Thin Layer Chromatography of lipids Assignment Example | Topics and Well Written Essays - 250 words

Thin Layer Chromatography of lipids - Assignment Example The material will dissolve in the solvent and move up the solvent front. (a) A Rf of 1 implies that the compound moved the same distance as the hexane solvent. Therefore, the analyzed compound was highly non-polar because it travelled the same distance as hexane, which is a non-polar solvent. (b) Ethyl acetate is a polar solvent. Mixing it with hexane will reduce the hydrophobic nature of hexane (Nollet & Toldra, 2012). Consequently, the Rf value of the same compound will reduce since it will not fully dissolve in the mixture of hexane and ethyl acetate. The TLC system for phospholipase D reaction will include the enzyme itself, phosphatidylcholine and ethyl acetate as the polar solvent. Phospholipase D will hydrolyze the phosphatidylcholine to phosphatidic acid and choline. Choline is more polar compared to phosphatidic acid; hence, it will move further than the acid. Molybdenum spray will be used to monitor the movement of the separated material. At 2 minute, there is little hydrolysis, which then increases to the sixth minute. Standard phosphatidic will be used as a control in the